Phaeohyphomycosis due to exophiala in aquarium-housed lumpfish (Cyclopterus lumpus): clinical diagnosis and description
By Colin T. McDermott, Charles J. Innis, Akinyi C. Nyaoke , Kathryn A. Tuxbury, Julie M. Cavin, E. Scott Weber, Deana Edmunds, Stéphane Lair, Jill V. Spangenberg, Amy L. Hancock-Ronemus, Catherine A. Hadfield, Leigh A. Clayton, Thomas B. Waltzek, Connie F. Cañete-Gibas, Nathan P. Wiederhold, Salvatore Frasca, Jr.
Originally published in Pathogens in November 2022
Abstract
Phaeohyphomycosis caused by Exophiala species represents an important disease of concern for farmed and aquarium-housed fish. The objective of this study was to summarize the clinical findings and diagnosis of Exophiala infections in aquarium-housed Cyclopterus lumpus. Clinical records and postmortem pathology reports were reviewed for 15 individuals from 5 public aquaria in the United States and Canada from 2007 to 2015. Fish most commonly presented with cutaneous ulcers and progressive clinical decline despite topical or systemic antifungal therapy. Antemortem fungal culture of cutaneous lesions resulted in colonial growth for 7/12 samples from 8 individuals. Amplification of the internal transcribed spacer region (ITS) of nuclear rDNA identified Exophiala angulospora or Exophiala aquamarina in four samples from three individuals. Postmortem histopathologic findings were consistent with phaeohyphomycosis, with lesions most commonly found in the integument (11/15), gill (9/15), or kidney (9/15) and evidence of fungal angioinvasion and dissemination. DNA extraction and subsequent ITS sequencing from formalin-fixed paraffin-embedded tissues of seven individuals identified E. angulospora, E. aquamarina, or Cyphellophora sp. in four individuals. Lesion description, distribution, and Exophiala spp. identifications were similar to those reported in farmed C. lumpus. Antemortem clinical and diagnostic findings of phaeohyphomycosis attributable to several species of Exophiala provide insight on the progression of Exophiala infections in lumpfish that may contribute to management of the species in public aquaria and under culture conditions.
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